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atcc tib 202  (ATCC)


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    ATCC atcc tib 202
    Atcc Tib 202, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 21248 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 99 stars, based on 21248 article reviews
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    Cytokines secreted by THP-1 cells. THP-1 cells were stimulated with PRN − , FHA − , FHA − PRN − , and WT isolates at an MOI of 10 or left unstimulated for 24, 48, and 72 h. Production of (A) IL-1β, (B) IL-6, (C) IL-12, (D) MCP-1, (E) CXCL2, and (F) G-CSF in the supernatant was determined by ELISA ( n = 3 ∼ 4). Results are shown as means and standard deviations. Brackets indicate individual comparisons of each mutant strain versus the WT control. Statistical significance was determined using two-way ANOVA followed by Dunnett’s multiple comparisons test; * p < 0.05; ** p < 0.01; *** p < 0.001; **** p < 0.0001; ns, not significant.

    Journal: Emerging Microbes & Infections

    Article Title: Pathogenicity and vaccine protection of circulating pertactin- and filamentous hemagglutinin-deficient Bordetella pertussis strains

    doi: 10.1080/22221751.2026.2640283

    Figure Lengend Snippet: Cytokines secreted by THP-1 cells. THP-1 cells were stimulated with PRN − , FHA − , FHA − PRN − , and WT isolates at an MOI of 10 or left unstimulated for 24, 48, and 72 h. Production of (A) IL-1β, (B) IL-6, (C) IL-12, (D) MCP-1, (E) CXCL2, and (F) G-CSF in the supernatant was determined by ELISA ( n = 3 ∼ 4). Results are shown as means and standard deviations. Brackets indicate individual comparisons of each mutant strain versus the WT control. Statistical significance was determined using two-way ANOVA followed by Dunnett’s multiple comparisons test; * p < 0.05; ** p < 0.01; *** p < 0.001; **** p < 0.0001; ns, not significant.

    Article Snippet: THP-1 cells (ATCC TIB 202; Supplementary Table 3) were seeded in 12-well (8.0 × 10 5 cells/well) culture plates (712012; NEST, China) and incubated overnight in RPMI 1640 medium (C11875500BT; Gibco, USA) supplemented with 2% FBS without antibiotics.

    Techniques: Enzyme-linked Immunosorbent Assay, Mutagenesis, Control

    Bacterial infection induced cell death in THP-1 cells. (A) Cell death induced in THP-1 cells following infection with PRN − , FHA − , FHA − PRN − , and WT isolates at 24, 48, and 72 h, with four cell populations: Q1 (cell death, FITC annexin V negative and PI positive); Q2 (cells undergoing late apoptosis or cell death, FITC annexin V and PI positive); Q3 (early apoptotic cells, FITC annexin V positive and PI negative); and Q4 (viable cells, FITC annexin V and PI negative) ( n = 3 ∼ 4). (B) Statistical analysis of early apoptosis in THP-1 cells ( n = 3 ∼ 4). (C) Statistical analysis of late apoptosis and death in THP-1 cells ( n = 3 ∼ 4). (D) Statistical analysis of total apoptosis and cell death in THP-1 cells ( n = 3 ∼ 4). Results are shown as means and standard deviations. Brackets indicate individual comparisons of each mutant strain versus the WT control. Statistical significance was determined using two-way ANOVA followed by Dunnett’s multiple comparisons test; * p < 0.05; ** p < 0.01; *** p < 0.001; **** p < 0.0001; ns, not significant.

    Journal: Emerging Microbes & Infections

    Article Title: Pathogenicity and vaccine protection of circulating pertactin- and filamentous hemagglutinin-deficient Bordetella pertussis strains

    doi: 10.1080/22221751.2026.2640283

    Figure Lengend Snippet: Bacterial infection induced cell death in THP-1 cells. (A) Cell death induced in THP-1 cells following infection with PRN − , FHA − , FHA − PRN − , and WT isolates at 24, 48, and 72 h, with four cell populations: Q1 (cell death, FITC annexin V negative and PI positive); Q2 (cells undergoing late apoptosis or cell death, FITC annexin V and PI positive); Q3 (early apoptotic cells, FITC annexin V positive and PI negative); and Q4 (viable cells, FITC annexin V and PI negative) ( n = 3 ∼ 4). (B) Statistical analysis of early apoptosis in THP-1 cells ( n = 3 ∼ 4). (C) Statistical analysis of late apoptosis and death in THP-1 cells ( n = 3 ∼ 4). (D) Statistical analysis of total apoptosis and cell death in THP-1 cells ( n = 3 ∼ 4). Results are shown as means and standard deviations. Brackets indicate individual comparisons of each mutant strain versus the WT control. Statistical significance was determined using two-way ANOVA followed by Dunnett’s multiple comparisons test; * p < 0.05; ** p < 0.01; *** p < 0.001; **** p < 0.0001; ns, not significant.

    Article Snippet: THP-1 cells (ATCC TIB 202; Supplementary Table 3) were seeded in 12-well (8.0 × 10 5 cells/well) culture plates (712012; NEST, China) and incubated overnight in RPMI 1640 medium (C11875500BT; Gibco, USA) supplemented with 2% FBS without antibiotics.

    Techniques: Infection, Mutagenesis, Control